CUSTOM ITEM: HPLC Column, Bidentate C8 300, 5um, 4.6mm ID x 250mm Length, 300A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑25P‑3M Cogent Bidentate C8™ 300 HPLC Column is a custom‑manufactured, long‑format wide‑pore reversed‑phase column designed for analytical separations requiring maximum resolution and extended retention for macromolecules. This column is particularly well suited for proteins, peptides, polysaccharides, and other high‑molecular‑weight analytes where conventional columns fail to provide adequate separation. Built on Cogent TYPE‑C™ silica hydride technology, it delivers excellent selectivity, faster equilibration, and extremely robust column lifetime under demanding analytical conditions.
The stationary phase utilizes an aliphatic C8 hydrocarbon chain bonded via direct silicon–carbon bonds to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than traditional wide‑pore C8 columns, yet better balanced than C18 for large biomolecules—providing strong yet controlled retention without excessive adsorption. The 300 Å pore architecture ensures proper accessibility for macromolecules, improving peak shape, mass transfer, and resolution.
Unlike standard 300 Å silica‑based phases, Bidentate C8™ 300 can retain compounds that often exhibit weak or inconsistent retention on traditional columns. It can also retain select hydrophilic macromolecules, particularly when used in HILIC‑like conditions, offering a broader method‑development window. Additionally, the column is stable at very low pH (below pH 2), enabling aggressive conditions not tolerated by conventional silica columns.
The 4.6 mm ID × 250 mm length configuration provides the maximum plate count and resolving power within the wide‑pore C8™ 300 family. This format is chosen when closely related macromolecules must be fully resolved or when longer retention times improve separation of complex biomolecular mixtures.
Why Choose the 4.6 × 250 mm Bidentate C8™ 300 Format
This configuration is typically selected when:
Maximum resolution is required for macromolecules
Shorter wide‑pore columns (50–150 mm) do not adequately separate critical species
Complex mixtures of proteins or polysaccharides require extended retention
Wide‑pore C18 causes excessive adsorption or peak distortion
Low‑pH conditions are necessary for method performance
The combination of long column length, wide‑pore architecture, and TYPE‑C™ silica hydride chemistry delivers a level of separation and robustness beyond conventional wide‑pore silica columns.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Direct Si–C bonding for exceptional durability
Reduced silanol activity for cleaner peak shapes
Faster equilibration than conventional wide‑pore silica columns
Bidentate C8™ 300 Bonding
Enhanced hydrophobicity vs standard C8
Balanced retention for macromolecules
Avoids excessive adsorption seen with wide‑pore C18
Wide‑Pore (300 Å) Architecture
Optimized for proteins, peptides, and polysaccharides
Improved mass transfer and chromatographic efficiency
Supports large‑molecule separations
Maximum‑Resolution Analytical Geometry
4.6 mm ID × 250 mm length
Highest plate count in the C8™ 300 lineup
Ideal for resolving closely related biomolecular species
Broad Method Compatibility
Stable at very low pH (below pH 2)
Compatible with aqueous‑rich mobile phases
Supports HILIC‑like operation for select macromolecules
Typical Applications
Protein and peptide separations
Polysaccharide and biomolecule analysis
High‑resolution LC‑MS and UV detection workflows
Wide‑pore reversed‑phase chromatography
Complex biomolecule mixture analysis
Low‑pH macromolecule methods
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable